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目的:本文旨在评估与代谢相关的葡萄糖转运体1(GLUT-1)、单羧酸转运体4(MCT-4)和碳酸酐酶9(CAⅨ)在喉鳞状细胞癌中的表达及临床意义。方法:收集经手术切除的40例喉鳞状细胞癌组织及10例癌旁组织,并对临床资料进行整理分析。应用免疫组织化学方法检测GLUT-1、MCT-4与CAⅨ在40例喉鳞状细胞癌和10例癌旁组织中的表达情况,评估它们与临床病理学资料的关系。所有数据采用SPSS21.0统计学软件进行分析。结果:在40例喉鳞状细胞癌组织中GLUT-1、MCT-4和CAⅨ的阳性表达率分别为72.5%、75.0%和55.0%。其中,MCT-4在正常组织中表达较低或几乎不表达,而GLUT-1在正常组织中具有干细胞活性的基底细胞层高表达。GLUT-1、MCT-4和CAⅨ的阳性表达与病理分级密切相关(均P<0.05),其中低分化的喉鳞状细胞癌中的阳性表达率远高于高分化组。此外,在40例喉鳞状细胞癌组织中MCT-4和CAⅨ的阳性表达和TNM分期相关,差异有统计学意义(均P<0.05)。MCT-4和CAⅨ在Ⅲ~Ⅳ期的表达率远高于Ⅰ~Ⅱ期。GLUT-1、MCT-4和CAⅨ的表达与患者的年龄、吸烟量及有无淋巴结转移及肿瘤的位置无关(P>0.05),GLUT-1、MCT-4和CAⅨ呈正相关。结论:葡萄糖摄取与酸性微环境在喉鳞状细胞癌代谢重塑和肿瘤的侵袭及演进密切相关,因此,在缺氧的微环境下靶向与代谢相关的蛋白质或许是治疗侵袭性肿瘤的有效策略。
OBJECTIVE: The purpose of this study was to evaluate the expression of GLUT-1, MCT-4 and CAⅨ in laryngeal squamous cell carcinoma and its clinical significance significance. Methods: Surgical resection of 40 cases of laryngeal squamous cell carcinoma tissue and 10 cases of adjacent tissues were collected, and the clinical data were analyzed. Immunohistochemistry was used to detect the expression of GLUT-1, MCT-4 and CAⅨ in 40 cases of laryngeal squamous cell carcinoma and 10 cases of paracancerous tissues, and their relationship with clinicopathological data was evaluated. All data were analyzed using SPSS 21.0 statistical software. Results: The positive rates of GLUT-1, MCT-4 and CAⅨ in 40 cases of laryngeal squamous cell carcinoma were 72.5%, 75.0% and 55.0%, respectively. Among them, MCT-4 is less or almost not expressed in normal tissues, while GLUT-1 is highly expressed in basal cell layers with stem cell activity in normal tissues. The positive expression of GLUT-1, MCT-4 and CAⅨ was closely related to the pathological grade (all P <0.05). The positive expression rate of poorly differentiated laryngeal squamous cell carcinoma was much higher than that of well-differentiated group. In addition, the positive expression of MCT-4 and CAⅨ in 40 laryngeal squamous cell carcinomas correlated with the TNM stage (P <0.05). The expression rates of MCT-4 and CAⅨ in stage Ⅲ ~ Ⅳ were much higher than those in stage Ⅰ ~ Ⅱ. The expression of GLUT-1, MCT-4 and CAⅨ was not related to the age of patients, smoking and lymph node metastasis and the location of tumor (P> 0.05). There was a positive correlation between GLUT-1, MCT-4 and CAIX. CONCLUSION: Glucose uptake is closely related to the metabolic remodeling of acidic squamous cell carcinoma and the invasion and evolution of the tumor in the laryngeal squamous cell carcinoma. Therefore, it is possible to target metabolic-related proteins in the hypoxic microenvironment for the treatment of aggressive tumors Strategy.