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目的采用人工接种羊源细粒棘球绦虫原头蚴感染法建立小鼠与兔包虫病动物模型。方法 6周龄昆明小鼠经皮穿刺腹腔内接种羊源细粒棘球绦虫原头蚴悬液,新西兰大白兔于腹部手术后肝脏接种羊源细粒棘球绦虫原头蚴悬液。接种原头蚴6个月后剖检动物,观察小鼠腹腔和新西兰大白兔肝脏内包虫囊生长情况。结果接种羊源细粒棘球绦虫原头蚴6个月后,小鼠腹腔内包囊生成率为95%,新西兰大白兔肝内包囊生成率为50%。光镜观察见在小鼠腹腔和兔肝脏形成的囊泡具有与羊肝脏棘球蚴囊壁类似的类上皮细胞层和板层状结构。3种动物体内棘球蚴均有原头蚴。结论以羊源细粒棘球绦虫原头蚴悬液接种昆明小鼠腹腔和新西兰大白兔肝脏,可以建立小鼠和兔包虫病动物模型。
Objective To establish an animal model of mouse and rabbit echinococcosis by using artificial inoculation of the original echinococcus granulosus inoculated with sheep. Methods Kunming mice (6 weeks old) were inoculated intraperitoneally with Prolactinoma of primates of E.granulosus, and New Zealand white rabbits were inoculated with Prolactinoma of Echinococcus granulosus in the liver after abdominal surgery. Animals were inoculated 6 months after inoculation of protozoospermia to observe the growth of internal hydatid cysts in the abdominal cavity of mice and New Zealand white rabbits. Results Six months after the inoculation of protozoa of Echinococcus granulosus, the incidence of cyst formation in peritoneal cavity of mice was 95%, and that of New Zealand white rabbits was 50%. Light microscopy showed that the vesicles formed in the peritoneal cavity and rabbit liver of mice exhibited epithelial and lamellar structures similar to those of hydatid cyst in sheep liver. Echinococcosis was found in all 3 species of animals. Conclusion The mouse and rabbit echinococcosis animal models can be established by inoculating the protozoa of Echinococcus granulosus suspension in the peritoneal cavity of Kunming mice and the liver of New Zealand white rabbits.