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目的检测乙型肝炎病毒相关性肾炎(HBV-GN)患者的HBV基因型,探讨HBV S区、P区变异与HBV-GN病情变化的相关性。方法对10例确诊HBV-GN患者取血,抽提全基因组DNA,DNA测序仪检测HBV P区、S区序列,进行病毒基因分型,并查找突变位点。结果 10例HBV-GN患者检出HBV基因型C型9例(占90%),B型1例(占10%);出现YVDD变异1例,同义突变1例,各占10%。结论本组HBV基因型以C型为主,发生变异少,未发现HBV变异与HBV-GN病情变化的相关性。
Objective To detect the HBV genotypes in patients with hepatitis B virus associated nephritis (HBV-GN) and to explore the relationship between HBV S, P region mutations and the changes of HBV-GN. Methods Totally 10 patients with HBV-GN were enrolled in the study. Total DNA was extracted and DNA sequencing was performed to detect the HBV P-region and S-region. The virus was genotyped and the mutation sites were searched. Results Nine cases (90%) of HBV genotype C and one case of type B (10%) were detected in 10 cases of HBV-GN. One case of YVDD mutation and one case of synonymous mutation were detected in 10 cases. Conclusion The genotypes of HBV in this group are mainly C type with few changes. No correlation between HBV mutation and HBV-GN status was found.