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应用HPLC法,以黄芩甙、黄芩甙元为对照品,黄酮为内标,测定黄芩中黄芩甙和黄芩甙元的含量。实验表明,按确定的色谱条件,黄芩甙进样量0.15～0.78μg,黄芩甙元进样0.10～0.40μg,均可得良好线性关系。通过试验,选择了样品提取方法和酶解时间。本法加样回收率为100.90％,重现性试验CV=2.46％。测定了11批样品,并作酶解前后对比,可确定黄芩是否经过杀酶及杀酶保甙的效果。为考察黄芩的切制工艺及质量提供了较为先进的检测手段和准确、灵敏、快速的方法。
The contents of Astragalus membranaceus and Astragalus membranaceus in Astragalus membranaceus were determined by HPLC with Huangqi and Huangqiong as the reference and flavonoids as the internal standard. The experiment shows that according to the determined chromatographic conditions, the injection amount of Radix Astragali is 0.15-0.78 μg and the Radix Astragali is injected with 0.10-0.40 μg, which can obtain a good linear relationship. Through the experiment, the sample extraction method and the enzymolysis time were selected. The recovery rate of this method is 100.90%, and the reproducibility test CV=2.46%. The 11 batches of samples were determined and compared before and after enzymatic hydrolysis to determine if the jaundice had been rancidally and catalyzed by enzymes. To provide a more advanced detection method and an accurate, sensitive, and rapid method for the inspection of the cutting process and quality of Astragalus membranaceus.