论文部分内容阅读
为研究人体对霍乱弧菌病原感染后血清产生霍乱抗毒素的时间及其维持多久,以便提供血清流行病学调查的参考数据和评价,作者对31例北美洲人志愿者作了人工霍乱弧菌口服试验。志愿者一次或多次摄入10~6个菌体,并以小苏打或食物冲淡胃酸。病原菌株包括古典稻叶型569B,古典小川型395,El Tor稻叶型P27459和N16961,El Tor小川型N15870,N18117和E7946。以微量法酶联免疫吸附试验(ELISA)检测血清IgG的抗毒素并根据标本比色的光密密(opticaldensity,O.D)为结果判断标准。结果:31例中7例(23%)粪便培养阳性,但未腹泻;18例出现中型腹泻(排便最平均为1.5升);6例为重型腹泻(排便量平
In order to study how long the human cholera antitoxin is produced in the serum after the onset of V. cholerae pathogen infection and how long it has been maintained in order to provide reference data and evaluation of serological epidemiological investigations, the authors orally administered 31 V. North American volunteers with V. cholerae test. One or more volunteers intake of 10 to 6 bacteria, and sodium bicarbonate or food to dilute the stomach acid. Pathogenic strains include classical rice leaf type 569B, classical Ogawa type 395, El Tor rice leaf type P27459 and N16961, El Tor Ogawa type N15870, N18117 and E7946. Serum IgG antitoxins were detected by micro-enzyme-linked immunosorbent assay (ELISA) and judged as a result based on the optical density (O.D) of the specimen colorimetric. RESULTS: Of the 31 cases, 7 (23%) had fecal culture positive but not diarrhea; 18 had middle-sized diarrhea (with an average defecation of 1.5 liters); 6 were severe diarrhea