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[目的]利用生物信息学方法预测绿脓杆菌外膜蛋白OprF的理化性质、高级结构和细胞表位。[方法]采用在线软件预测OprF蛋白的理化性质;Signal P 4.1软件预测OprF信号肽序列;利用TMHMM软件预测ACFA蛋白跨膜结构;SOPMA服务器预测蛋白的二级结构;Swiss-Model程序预测OprF三维结构;综合ABCpred与Bepi Pred方案预测OprF的B细胞表位;运用神经网络法预测OprF的CTL表位;使用MHC-Ⅱ类分子结合肽程序预测OprF的Th细胞表位。[结果]OprF为亲水性蛋白;1~24位氨基酸为信号肽序列;存在多个酶切位点;无跨膜结构并定位于细胞膜外;二级结构中含无规则卷曲34.36%、α-螺旋31.90%、β-转角11.66%、β-片层22.09%;并可能存在3个B细胞表位、2个CTL表位、4个Th细胞表位。[结论]系统分析了OprF蛋白的理化性质、信号肽、跨膜结构、二级与三级结构,以及B、T细胞抗原表位。
[Objective] The bioinformatics method was used to predict the physicochemical properties, higher structure and cell epitopes of OprF from Pseudomonas aeruginosa. [Method] The physicochemical properties of OprF protein were predicted by on-line software. The OprF signal peptide sequence was predicted by Signal P 4.1 software. The transmembrane structure of ACFA protein was predicted by TMHMM software. The secondary structure of protein was predicted by SOPMA server. ; Predicting the B cell epitopes of OprF by combining ABCpred and Bepi Pred regimens; predicting the CTL epitopes of OprF by using neural network; and predicting the Th cell epitope of OprF by using MHC class II molecule binding peptide programs. [Results] OprF was a hydrophilic protein; amino acids 1-24 was signal peptide sequence; there were multiple restriction sites; no transmembrane structure was located outside the cell membrane; secondary structure contained 34.36% - Spiral 31.90%, β-turn 11.66%, β-sheet 22.09%; and there may be three B cell epitopes, two CTL epitopes, four Th cell epitopes. [Conclusion] The physicochemical properties, signal peptide, transmembrane structure, secondary and tertiary structure of OprF protein and antigenic epitopes of B and T cells were systematically analyzed.