论文部分内容阅读
目的 :观察中药清肠栓对实验性溃疡性结肠炎大鼠血浆TXB2 和 6 -Keto -PGF1α的影响 ,探讨其作用机理。方法 ;用三硝基苯磺酸复制大鼠实验性溃疡性结肠炎模型 ,随机分为清肠栓大小剂量组、西药组、模型组、空白对照组和正常对照组 ,治疗 14天后处死 ,腹主动脉抽血 5ml,放免法测定血浆TXB2 和 6 -Keto -PGF1α含量。结果 :模型、空白对照组血浆TXB2 较正常组明显升高 ,6 -Keto -PGF1α降低 ,TXB2 / 6 -Keto -PGF1α比值升高 ;各药物治疗组血浆TXB2 、6 -Keto -PGF1α、TXB2 / 6 -Keto -PGF1α比值均接近正常组 ,与模型、空白对照组比较有显著差异 ;西药、中药大、小剂量组之间各项指标无显著差异。结论 :中药清肠栓治疗溃疡性结肠炎的作用机理与稳定细胞膜 ,降低磷脂酶活性 ,降低血浆TXB2 ,升高 6 -keto -PGF1α,降低TXB2 / 6 -Keto -PGF1α比值 ,改善肠黏膜血液循环有关。
Objective: To observe the effect of Qingchang Suppository on plasma TXB2 and 6-Keto-PGF1α in rats with experimental ulcerative colitis, and to explore its mechanism of action. METHODS: Rat model of experimental ulcerative colitis was duplicated with trinitrobenzene sulfonic acid and randomly divided into large and small doses of Qingchang suppository, western medicine group, model group, blank control group and normal control group. The rats were sacrificed after 14 days of treatment. The aorta was drawn 5 ml and plasma TXB2 and 6-Keto-PGF1α levels were determined by radioimmunoassay. Results: Plasma TXB2 levels in the model and blank control group were significantly higher than those in the normal group, 6-Keto-PGF1α was decreased, and TXB2 / 6-Keto-PGF1α ratio was increased. Plasma TXB2, 6-Keto-PGF1α, and TXB2 / 6 in each drug treatment group. The ratio of Keto to PGF1α was close to the normal group, and there was a significant difference between the model and the blank control group. There was no significant difference between the Western medicine and the large and small doses of traditional Chinese medicine. Conclusion: The effect of Qingchang suppository in the treatment of ulcerative colitis and the stabilization of cell membrane, decrease of phospholipase activity, decrease of plasma TXB2, increase of 6-keto-PGF1α, decrease of TXB2 / 6-Keto-PGF1α ratio, and improvement of intestinal mucosal blood circulation related.