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将牛肺动脉内皮细胞培养于明胶处理后的微孔滤膜,形成致密融合单层后将滤膜上细胞单层装入我们设计的灌流装置,可以简便快速地测定内皮单层对小分子物质的滤过系数(Kf)和对蛋白质的渗透压反射系数(σ)。用Hanks平衡盐溶液(HBSS)和0.5g/dl白蛋白溶液灌流对照组内皮单层时,收集液流量(Jv)分别是24.6±
Bovine pulmonary artery endothelial cells cultured in a microporous membrane after gelatin treatment to form a dense fusion monolayer filter cell monolayer into our design of perfusion device can be easily and quickly determine the endothelial monolayer of small molecule substances Filtration coefficient (Kf) and osmotic pressure reflection coefficient (σ) on the protein. When the control monolayers were perfused with Hanks Balanced Salt Solution (HBSS) and 0.5 g / dl albumin solution, the flow rates (Jv) were 24.6 ±