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1986年6月至1987年12月,我科共做尖锐湿疣活组织检查80例。诊断根据病史,大体表现和组织学检查。光镜下每例均查见凹空细胞。(Koilocyte)30例作了人乳头瘤病毒抗原(HPV—Ag)检测,采用PAP免疫酶染色法(DAKO kit)。12例检测出HPV—Ag,阳性率40％。阳性细胞主要见于表层和棘层上部的凹空细胞,表现为细胞核内呈棕褐色颗粒,细胞浆内未见棕褐色颗粒。各例阳性细胞数量差异较大,阳性率与取材大小和数量有较大系系。HPV—Ag阳性的凹空细胞与HPV—Ag阴性的凹空细胞,光镜下形态无明显差异。15例取新鲜组织按电镜常规处理,制片,电镜下5例找到病毒颗粒,阳性率33.3％。均见于表层和棘层最上部细胞的核内。病毒颗粒呈球形,直径50-55nm,有致密核心和衣壳,无包膜,典型者排列呈结晶状图1。凹空细胞较大,核增大,核膜欠规则,一些细
June 1986 to December 1987, our department made a total of 80 cases of genital warts biopsy. Diagnosis based on medical history, general performance and histological examination. Each case under light microscopy see concave cells. (Koilocyte) 30 cases of human papillomavirus antigen (HPV-Ag) detection, the use of PAP immune enzyme staining (DAKO kit). 12 cases detected HPV-Ag, the positive rate of 40%. Positive cells are mainly found in the surface of the concave layer of the upper part of the stratum spinosum cells, showing brown granules in the nucleus, no brown granules in the cytoplasm. The number of positive cases in each case is quite different. The positive rate is related to the size and number of samples. There was no significant difference in morphology between HPV-Ag positive pouch cells and HPV-Ag negative pouch cells. Fifteen cases of fresh tissue were treated by electron microscope, and 5 cases of virus particles were found under tablet and electron microscope. The positive rate was 33.3%. Are found in the surface and spinous layer of the uppermost cells of the nucleus. Virus particles were spherical, diameter 50-55nm, a dense core and capsid, no capsule, the typical arrangement of crystalline Figure 1. Null empty cell larger, nuclear increases, the nuclear membrane less regular, some fine