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Extracellular polymeric substances (EPS) produced by microorganisms represent biological macromolecules with unfathomable potentials and they are required to be explored further for their potential application as a bioflocculant in various wastewater sludge treatment Although several studies already exist on biosynthetic pathways of different classical biopolymers like alginate and xanthan,no dedicated studies are available for EPS in sludge.This review highlights the EPS composition,functionality,and biodegradability for its potential use as a carbon source for production of other metabolites.Furthermore,the effect of various extraction methods (physical and chemical) on compositional,structural,physical and functional properties of microbial EPS has been addressed.The vital knowledge of the effect of extraction method on various important attributes of EPS can help to choose the suitable extraction method depending upon the intended use of EPS.The possible use of different molecular biological techniques for enhanced production of desired EPS was summarized.