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目的模拟高原条件下建立SD大鼠牙周炎动物模型,检测其龈沟液中C-反应蛋白(C-reactive protein,CRP)变化,以探索高原牙周炎发病机制。方法选取60只SD大鼠,雌雄各半,采用随机数字表法分为6组:平原对照组(A组)、平原牙周炎组(B组)、高原对照组(C组)、高原牙周炎组(D组)、平原牙周炎模型判定组(E组)、高原牙周炎模型判定组(F组),每组10只。B组和E组,D组和F组分别建立平原牙周炎和高原牙周炎动物模型,8周后E、F组判定造模成功,采用ELISA法检测剩余4组大鼠龈沟液中CRP浓度变化。结果A、B、C、D组龈沟液中CRP浓度分别为(6.33±1.58)、(13.47±1.98)、(7.76±1.84)、(16.26±2.14)μg/ml,B组与A组,D组与C组,C组与A组,D组与B组比较差异均有统计学意义(P<0.05),其中以D组升高最为显著。结论高原牙周炎组大鼠龈沟液中的CRP水平高于平原牙周炎组,提示高原环境下牙周炎的病变程度更严重。
Objective To establish an animal model of periodontitis in SD rats under simulated plateau conditions and measure the changes of C-reactive protein (CRP) in gingival crevicular fluid to explore the pathogenesis of plateau periodontitis. Methods Sixty male Sprague-Dawley rats were randomly divided into 6 groups: control group (group A), placenta periodontitis group (group B), plateau control group (group C), plateau teeth Week group (group D), plain periodontitis model group (group E), plateau periodontitis model group (group F), with 10 rats in each group. Group B, group E, group D and group F were respectively established animal models of periodontitis and plateau periodontitis. After 8 weeks, the rats in groups E and F were successful in establishing models. ELISA was used to detect the remaining 4 groups of rat gingival crevicular fluid CRP concentration changes. Results The CRP concentrations in GCF group were (6.33 ± 1.58), (13.47 ± 1.98), (7.76 ± 1.84) and (16.26 ± 2.14) μg / ml respectively in group A, B, There were significant differences between D group and C group, C group and A group, D group and B group (P <0.05), and the most significant increase was in D group. Conclusion The level of CRP in gingival crevicular fluid of rats in plateau periodontitis group is higher than that in periodontitis group, suggesting that the severity of periodontitis in plateau environment is more serious.