论文部分内容阅读
为区分动脉粥样硬化斑块和血管壁,给激光血管成形术的实时监控提供依据,研究了动脉粥样硬化斑块和血管壁的激光诱导荧光光谱(LIFS)和拉曼光谱。日本雄性大耳白兔15只,以高脂饲料喂养3个月,取主动脉弓作纵行切开,分别采用380 nm紫外激光和532 nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管壁的荧光光谱;采用532 nm绿激光作为激发光源诱导动脉粥样硬化斑块和血管壁的拉曼光谱。结果表明,380 nm紫外激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在416 nm处均有明显特征峰,但前者的强度显著高于后者;532 nm绿激光诱导的动脉粥样硬化斑块和血管壁的荧光光谱在800 nm处均有明显特征峰,但强度无明显差别;动脉粥样硬化斑块的拉曼光谱在3000 nm和3300 nm处存在明显的波峰和波谷,而血管壁的拉曼光谱曲线较光滑,无明显特征峰。说明380 nm紫外激光诱导的荧光光谱和拉曼光谱均有可能有效区分动脉粥样硬化斑块和血管壁,而532 nm绿激光诱导的荧光光谱不能有效区分动脉粥样硬化斑块和血管壁。
To distinguish between atherosclerotic plaque and vessel wall, laser angioplasty was used to provide a basis for real-time monitoring of laser-induced fluorescence (LIFS) and Raman spectra of atherosclerotic plaque and vessel wall. Fifteen male Japanese white rabbits were fed with high fat diet for 3 months. The aortic arch was longitudinally dissected. 380 nm UV laser and 532 nm green laser were used as excitation light sources to induce atherosclerotic plaque and vessel wall Fluorescence spectra of 532 nm green laser were used as excitation light sources to induce atherosclerotic plaque and vessel wall Raman spectra. The results showed that the fluorescence spectra of atherosclerotic plaque and the vascular wall induced by 380 nm ultraviolet laser at 416 nm all had obvious characteristic peaks, but the intensity of the former was significantly higher than that of the latter. The 532 nm green laser-induced atherosclerosis Fluorescence spectra of sclerosing plaque and blood vessel wall showed obvious characteristic peaks at 800 nm but no significant difference in intensity; Raman spectrum of atherosclerotic plaques showed obvious peaks and valleys at 3000 nm and 3300 nm The Raman spectrum of blood vessel wall is smooth, with no obvious characteristic peak. It is concluded that both fluorescence and Raman spectra induced by UV at 380 nm are effective for distinguishing between atherosclerotic plaque and vessel wall. However, the fluorescence spectrum induced by 532 nm green laser does not distinguish between atherosclerotic plaque and vessel wall.