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以十六烷基三甲基溴化铵为电渗流改向剂，建立了适合乙酰水杨酸制剂优化分离与定量分析的反向高效毛细管电泳法。电泳分离条件：熔融石英毛细管４０ｃｍ×５０μｍ，ｕｎｃｏａｔｅｄ（河北永年），缓冲液组成为０．０５ｍｏｌ／Ｌ磷酸盐溶液（ｐＨ５．０），含０．５ｍｍｏｌ／Ｌ电渗流改向剂和５．０ｍｍｏｌ／Ｌβ－环糊精，运行电压１１ｋＶ（－）→（＋），柱上检测ＵＶ２１０ｎｍ，０．０２ａｕｆｓ。以磺基水杨酸为内标，测得乙酰水杨酸浓度在０．０５～０．２５ｍｇ／ｍｌ范围内与样品峰面积比的线性关系良好，相关系数ｒ＝０．９９９４，平均回收率为９７．２２％，日内ＲＳＤ低于０．９６％，日间ＲＳＤ低于２．６％。
Using cetyltrimethylammonium bromide as the redirecting agent, a reversed-phase high performance capillary electrophoresis method was established for the optimal separation and quantitative analysis of acetylsalicylic acid. Electrophoresis separation conditions: fused silica capillary 40cm × 50μm, uncoated (Hebei Yongnian), the buffer composition 0.05mol / L phosphate solution (pH5.0), containing 0.5mmol / L electroosmotic flow redirecting agent and 5.0mmol / Lβ-cyclodextrin, operating voltage 11kV (-) → (+), UV 210nm detection column, 0.02aufs. With sulfosalicylic acid as the internal standard, the linear relationship between the peak area ratio of acetylsalicylic acid in the range of 0.05-0.25 mg / ml was obtained, the correlation coefficient was 0.9994, the average recovery 97.22%, intraday RSD less than 0.96%, daytime RSD less than 2.6%.