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目的:对深圳市龙岗区2004~2008年间分离到的伤寒沙门菌进行同源性分析。方法:收集深圳市龙岗区2004~2008年伤寒沙门菌并用生化和血清学方法进行鉴定,伤寒沙门菌基因组经限制性内切酶XbaI酶切后,采用脉冲场电泳(PFGE)获得电泳图谱,再利用BioNumerics软件对电泳图谱进行同源性分析。结果:在深圳市龙岗区2004~2008年间共分离到32株伤寒沙门菌。BioNumerics分析结果显示,共有24个不同的PFGE带型出现,除一株以外其余31株分布于6个相似性在85%以上的簇内。不同年份之间既有交叉又有差异。结论:深圳市龙岗区可能存在6个伤寒沙门菌的流行克隆,这些克隆传播可能是本区伤寒沙门菌发生的主要原因,因此公共卫生建设仍需加强。PFGE指纹图谱为本区伤寒沙门菌分子分型网络监测打下了良好的基础,为伤寒沙门菌所致疾病及时主动监测和传染来源追踪提供了技术支持。
Objective: To analyze the homology of Salmonella typhi isolated from 2004 to 2008 in Longgang District, Shenzhen. Methods: Salmonella typhi was collected from 2004 to 2008 in Longgang District of Shenzhen City. The Salmonella typhi was identified by biochemical and serological methods. The Salmonella typhi genome was digested with restriction endonuclease XbaI and then electrophorogram was obtained by pulse field electrophoresis (PFGE) Homology analysis of electrophoresis patterns was performed using BioNumerics software. Results: A total of 32 Salmonella typhi strains were isolated from 2004 to 2008 in Longgang District, Shenzhen City. BioNumerics analysis showed that a total of 24 different PFGE bands appeared, with the exception of one strain and the remaining 31 distributed in 6 clusters of 85% similarity. There are differences and differences between different years. CONCLUSION: There may exist six epidemic clones of Salmonella typhi in Longgang district, Shenzhen. The clonal spread may be the main reason for the occurrence of Salmonella typhi in this area, so public health construction still needs to be strengthened. PFGE fingerprinting laid a good foundation for the molecular typing of Salmonella typhimurium in this area and provided technical support for the active surveillance and source tracking of the disease caused by Salmonella typhi.