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目的建立了紫外检测的快速灵敏的高效液相色谱法,用于测定犬血浆中的丹参素钠。方法血浆样品经液-液萃取后,以原儿茶酸为内标,采用Discovery C18色谱柱进行分离,HPLC测定犬血浆中的药物浓度。结果犬分别静脉滴注给予10、20、40 mg/kg丹参素钠后,AUC0-t、AUC0-∞、Cmax均与给药剂量呈线性相关。AUC0-t、AUC0-∞的相关系数分别为0.964、0.964。Cmax的相关系数为0.934 7;丹参素钠在犬体内的药动学过程符合二室模型。结论通过方法学确证,该方法可以应用于丹参素钠在犬血浆中的药物动力学研究。
Objective To establish a rapid and sensitive HPLC method for the determination of danshensu in the plasma of dogs. Methods Plasma samples were separated by liquid-liquid extraction using protocatechuic acid as the internal standard and were separated on a Discovery C18 column. The plasma concentration of the drug was measured by HPLC. Results The AUC0-t, AUC0-∞ and Cmax of 10,20 and 40 mg / kg Danshensu were intravenously administered to the dogs in a dose-dependent manner. AUC0-t, AUC0-∞ correlation coefficients were 0.964,0.964. The correlation coefficient of Cmax was 0.934 7. The pharmacokinetics of sodium danshensu in dogs was in accordance with the two-compartment model. Conclusion Through methodological confirmation, this method can be applied to the pharmacokinetics study of Danshensu sodium in canine plasma.