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目的：了解髓母细胞瘤发生、分化及其病理生物学行为，方法：４６ 例手术切除髓母细胞瘤组织用免疫组织化学方法对胶质酸性蛋白（ Ｇ Ｆ Ａ Ｐ）、中间丝结蛋白（ｖｉｍ ｅｎｔｉｎ）、神经纤维（ Ｎ Ｆ）、增殖细胞核抗原（ Ｐ Ｃ Ｎ Ａ）、抑癌基因ｐ５３ 及 Ｓ１００ 蛋白进行检测。结果： Ｇ Ｆ Ａ Ｐ、ｖｉｍ ｅｎｔｉｎ、 Ｐ Ｃ Ｎ Ａ、 Ｓ１００ 蛋白阳性率分别为 ７１．１１％ 、４３．４４％ 、９２．００％ 、５２．１７％ ，阳性指数分别为４６．２５‰、１７．００‰、８７５．４０‰、８．４２‰。 Ｎ Ｆ表达极弱，而ｐ５３ 几乎不表达。结论：髓母细胞瘤的发生可能与 ｐ５３ 抑癌基因突变无明显相关；髓母细胞瘤的多数肿瘤细胞向胶质细胞方向分化；髓母细胞生长迅速以及其高度恶性与 Ｐ Ｃ Ｎ Ａ 检测超常表达相一致。
Objective: To investigate the occurrence, differentiation and pathobiological behaviors of medulloblastoma. Methods: 46 cases of medulloblastoma were surgically resected with immunohistochemistry for glial acidic protein (GFPA), intermediate silk protein ( Vim entin), nerve fibers (NF), proliferating cell nuclear antigen (PCNA), tumor suppressor gene p53 and S-100 protein were detected. Results: The positive rates of G F A P, vim entin, P C N A and S100 proteins were 71.11%, 43.44%, 92.00% and 52.17%, respectively, and the positive indexes were 46.25. ‰, 17.00‰, 875.40‰, 8.42‰. The expression of NF is extremely weak, while p53 is hardly expressed. Conclusion: The occurrence of medulloblastoma may not be significantly associated with p53 tumor suppressor gene mutations; most of the tumor cells of medulloblastoma differentiate into glial cells; rapid growth of marrow cells and its high degree of malignancy and abnormal detection of P C N A The expression is consistent.