Cellular response to titanium discs coated with polyelectrolyte multilayer films

• 作 者: Jing Zhan Qiao-jie Luo Ying Hu
• 机 构: Department of Stomatology,Department of 0ral and Maxil ofacial Surgery
• 出 处: 矿物冶金与材料学报
• 发表日期: 2004年期
• 关键词: titanium surface modification polyelectrolytes multilayer films cellular respons
• 基金项目: This work was financially supported by the National Natural Science Foundation of China;the National Science-Technology Support Plan Project of China the Fundamental Research Funds for Central Un

The purpose of this study was to investigate the effects of polyelectrolyte multilayer (PEM) coatings on the biological behavior of titanium (Ti) substrates. Collagen typeΙ/hyaluronic acid (Col/HA) and chitosan/hyaluronic acid (Chi/HA) multilayer PEM coatings were in-troduced onto Ti substrates using layer-by-layer assembly. Contact angle instruments and quartz crystal microbalance were used for film characterization. The results obtained showed that both Col/HA and Chi/HA surfaces had high hydrophilicity and promoted cell adhesion in MC3T3-E1 pre-osteoblast and human gingival fibroblast cells. In addition, the synthesis of function-related proteins and gene expression levels in both MC3T3-E1 and fibroblast cells was higher for the Col/HA coating compared with the Chi/HA coating, indicating better cellu-lar response to the Col/HA coating.