论文部分内容阅读
一、方法本文推荐一个用角叉藻胶固定活微生物细胞的新方法。在无菌条件下,37℃,将4％角叉藻胶与预先培养好的微生物细胞混和。于20℃、温和搅拌条件下,将上述混和物滴加到2％氯化钾溶液中,形成包埋少量活细胞的角叉藻小珠。然后再将其置于适当的营养培养基中,于30℃、旋转摇床上,保温培养。这些被包埋的细胞在小珠中生长,并且将生长细胞固定。为了测定活细胞的数目,将角叉藻胶小珠溶解于无菌生理盐水中,于37℃、振荡15分钟,使成为细胞悬浮液。然后用稀释
First, the method This article recommends a new method of fixing live microbial cells with carrageenan. Under aseptic conditions, 4% carrageenan was mixed with precultured microbial cells at 37 ° C. The above mixture was added dropwise to a 2% potassium chloride solution at 20 ° C with gentle stirring to form carrageenan beads entrapped in a small amount of viable cells. Then placed in the appropriate nutrient medium, at 30 ℃, rotary shaker, incubation. These embedded cells grow in the beads and fix the growing cells. To determine the number of viable cells, the carrageenan beads were dissolved in sterile saline and shaken at 37 ° C for 15 minutes to become a cell suspension. Then dilute