论文部分内容阅读
【目的】明确辣木果荚腐病病原菌种类及其分类地位,为辣木果荚腐病防治提供理论依据。【方法】对云南省西双版纳州辣木种植基地感病、带黑色颗粒物病残体的辣木果荚进行症状观察、病原菌分离培养、致病性测定、显微形态特征观察、ITS和β-tubulin基因序列分析。【结果】辣木果荚腐病病原菌的分生孢子不分隔、透明,孢子顶端呈尖锥形弯曲,基部平截,具油滴,大小为15.7~22.1μm×2.5~4.3μm;厚垣孢子壁厚,呈黑褐色,簇生或成链状。该菌株ITS和β-tubulin基因的序列与Colletotrichum chlorophyti菌株IMI103806的同源性高达99%(ITS登录号:GU227894;TUB2登录号:GU228188)。多基因联合系统发育分析结果表明,供试菌株与兰生炭疽菌(C.chlorophyti)具有很近的遗传关系。【结论】兰生炭疽菌(C.chlorophyti)能侵染辣木引起果荚腐病,是为害辣木果荚的致病菌。
【Objective】 The purpose of this study was to determine the species and taxonomic status of pathogen of Moringa oleifera and to provide a theoretical basis for the prevention and treatment of Moringa oleifera. 【Method】 The symptoms, pathogens isolation and pathogenicity determination, microscopic morphological observation, ITS and β-tubulin were observed in Moringa pods infected with black particulate matter in the Moringa planted in Xishuangbanna Prefecture of Yunnan Province. Gene sequence analysis. 【Result】 The results showed that the conidia of the pathogen of Moringa rotundifolia were not separated and transparent. The top of the spore was curved conically, the base was truncated with oil droplets and the size was 15.7 ~ 22.1μm × 2.5 ~ 4.3μm. The chlamydospore Wall thickness, dark brown, tufted or in chains. The ITS and β-tubulin gene sequences of this strain shared 99% homology with Colletotrichum chlorophyti strain IMI103806 (ITS accession number: GU227894; TUB2 accession number: GU228188). The phylogenetic analysis of polygenes showed that there was a close genetic relationship between the tested strains and C. chlorophyt. 【Conclusion】 C. chlorophytum can infect Moringa oleifera-induced pod rot, which is the causative agent of Moringa oleifera.