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目的研究耐甲氧西林金黄色葡萄球菌对临床常用化学消毒剂抗力,为实际消毒提供参考。方法采用肉汤稀释法测定3种常用消毒剂对8株临床分离耐甲氧西林金黄色葡萄球菌最小抑菌浓度和最小杀菌浓度,同时与金黄色葡萄球菌标准株作平行比较。结果三氯异氰尿酸对8株临床分离的耐甲氧西林金黄色葡萄球菌的最小抑菌浓度范围为有效氯250～1000mg/L,最小杀菌浓度范围为500～2000mg/L;对金黄色葡萄球菌标准株的MIC为500mg/L,MBC为1000mg/L。碘伏对8株临床分离的耐甲氧西林金黄色葡萄球菌和标准株的MIC和MBC分别为25mg/L和50mg/L。戊二醛对8株临床分离的耐甲氧西林金黄色葡萄球菌和标准株MIC和MBC分别为31mg/L和62mg/L。结论临床分离的耐甲氧西林金黄色葡萄球菌对三氯异氰尿酸的抗力与标准株比较有所不同,但碘伏和戊二醛对耐甲氧西林金黄色葡萄球菌抗力与标准株一致。
Objective To study the resistance of methicillin-resistant Staphylococcus aureus to clinical common disinfectants and to provide reference for practical disinfection. Methods The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MIC) of three commonly used disinfectants against eight methicillin-resistant Staphylococcus aureus isolates were determined by broth dilution method and compared with Staphylococcus aureus standard strains. Results The minimum inhibitory concentrations of trichloroisocyanuric acid against eight clinical isolates of methicillin-resistant Staphylococcus aureus ranged from 250 mg / L to 1000 mg / L and the minimum bactericidal concentration ranged from 500 mg / L to 2000 mg / L. Cocci standard strain MIC 500mg / L, MBC 1000mg / L. The MIC and MBC of iodophor against 25 strains of methicillin-resistant Staphylococcus aureus and standard strains were 25 mg / L and 50 mg / L, respectively. Glutaraldehyde resistance to methicillin-resistant Staphylococcus aureus and standard strains MIC and MBC of 8 clinical isolates were 31mg / L and 62mg / L respectively. Conclusion The clinical isolates of methicillin-resistant Staphylococcus aureus are more resistant to TCA than the standard strains, but the resistance to methicillin-resistant Staphylococcus aureus by iodophor and glutaraldehyde is consistent with that of the standard strains.