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【目的】 探讨新生动物缺氧缺血性脑病（ ＨＩＥ） 脑皮层细胞凋亡的发生及其相关的诱导因素。【方法】 用琼脂电泳观察“ＤＮＡ 梯带”的出现，用流式细胞术（ＦＣＭ） 对脑皮层细胞凋亡及细胞内游离钙（ＩＦＣａ） 、抑癌基因ｐ５３表达水平进行动态研究。【结果】 缺氧缺血后４ｈｒ 新生猪同侧额顶叶脑皮层细胞未见明显凋亡，７２ｈｒ 后实验组凋亡率较对照组及尼莫通治疗组明显出现（ ｎ ＝ １５ ，１２ ，１３ ；－ｘ ±ｓ＝ ２７ ．５０ ±１６ ．２ ，３ ．２３ ±２ ．９３ ，９ ．１７ ±５ ．７６ ，Ｆ＝ １８ ．７５６ ４ ，Ｐ＜ ０ ．０００ １） ；ＨＩ 后４ｈｒ ＩＦＣａ 及ｐ５３ 基因阳性表达率明显增高（ ｎ ＝ １２ ，１２ ，１２ ；－ｘ ±ｓ ＝ ３４８ ．９６ ±３９ ．５９ ，１６０ ．９８ ±３７ ．０５ ，２６０ ．１８ ±６６ ．８１ ，Ｆ＝ ４１ ．１６８ １ ，Ｐ＜ ０ ．０００ １ ；－ｘ ±ｓ ＝ ６ ．０７ ±５ ．５５ ，１ ．１８ ±０ ．５３ ，３ ．１３ ±２ ．１８ ，Ｆ＝ ６ ．０７６ ７ ，Ｐ＝ ０ ．００５ ７） ，且两者间呈显著正相关（ ｒ ＝ ０ ．４９７ ７ ，Ｐ＝ ０ ．００２） ；ＨＩ 后７２ｈｒ 凋亡率与４ｈｒ ＩＦＣａ 水平呈显著正相关（ ｒ ＝ ０ ?
【Objective】 To investigate the occurrence and related factors of apoptosis in cortex of neonatal hypoxic-ischemic encephalopathy (HIE). 【Method】 The appearance of “DNA ladder” was observed by agarose electrophoresis. The apoptosis of cortical cells and the expression of intracellular free calcium (IFCa) and tumor suppressor gene p53 were studied by flow cytometry (FCM). 【Results】 No significant apoptosis occurred in ipsilateral frontal cortex of 4 h after hypoxia-ischemia. The apoptotic rate of experimental group was significantly higher than that of control group and nimotop group after 72 hr (n = 15, 12, 13; -x ± s = 27 .50 ± 16 .2, 3.23 ± 2 .93, 9 .17 ± 5 .76, F = 18 .756 4, P <.0001) And p53 gene positive expression rate was significantly higher (n = 12, 12, 12; -x ± s = 348.96 ± 39.59, 160.98 ± 37.05, 260.18 ± 66.81, F = 41. 168 1, P <0 .000 1; -x ± s = 6 .07 ± 5 .55, 1.18 ± 0 .53, 3.13 ± 2 .18, F = 6 .076 7, P = 0. 005 7), and there was a significant positive correlation between them (r = 0.497 7, P = 0 .0 02). The apoptotic rate at 72hr after HI was positively correlated with 4hr IFCa (r = 0?