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对43例分泌性中耳炎病人经中耳抽出物培养,使用单克隆抗体经荧光抗体技术证实6例存在衣原体包涵体,并经微量免疫荧光法证实病人血清中存在抗衣原体抗体。虽认为感染与分泌性中耳炎的发展有关,但20～50%中耳渗液培养无细菌生长,提示有病原或仍未查明细菌存在。本组6例从中耳抽出物中分离出衣原体,且有可察觉的衣原体抗体,IgG抗体增高4倍或更多(1∶16～1∶256)和/或IgM滴度为1∶16或更多。认为衣原体和病因有关。其中
Thirty - three patients with secretory otitis media were cultured in middle ear extract. The presence of chlamydial inclusion body in 6 patients was confirmed by the fluorescent antibody technique using monoclonal antibody. The presence of anti - Chlamydia antibody in serum of patients was confirmed by micro - immunofluorescence assay. Although the infection and the development of secretory otitis media that, but 20 to 50% of the middle ear exudate culture without bacteria, suggesting that there is no pathogen or bacterial existence. The group of 6 patients isolated from the middle ear extract Chlamydia, and there are detectable Chlamydia antibodies, IgG antibodies increased 4 times or more (1:16 ~ 1:256) and / or IgM titers of 1:16 or more many. Think chlamydia and cause related. among them