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原位杂交组织化学是近年发展起来的检测组织细胞中特异性DNA或RNA的技术。它是利用互补碱基对间特异性结合的特点,在已知DNA或RNA链上掺入一些标记物作为探针,去检测组织细胞中与它相应的DNA或RNA。最初一般应用DNA探针,此法比较成熟,探针也易制备,因此应用比较广泛,但由于它敏感性不高,因此在应用方面有它的局限性。RNA探针与DNA探针相比其敏感性是后者的十倍。由于单个细胞中神经肽DNA或RNA的拷贝数少,所以研究神经肽基因表达方面多用RNA探针。我们承Hoefler教
In situ hybridization histochemistry is a technique developed in recent years to detect specific DNA or RNA in tissue cells. It is the use of complementary base pairs between the specific binding characteristics of the known DNA or RNA chain splicing some markers as a probe to detect tissue cells corresponding to its DNA or RNA. The first general application of DNA probes, the method is relatively mature, the probe is also easy to prepare, it is widely used, but because of its sensitivity is not high, so its application has its limitations. RNA probes are ten times more sensitive to DNA probes than the latter. Due to the small copy number of neuropeptide DNA or RNA in a single cell, multi-use RNA probes for neuropeptide gene expression were studied. We accept Hoefler teach