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对从武汉地区免疫功能低下患者分离的１０６３株革兰氏阴性杆菌，用琼脂平皿稀释法测定１６种抗菌药物的最小抑菌浓度（ＭＩＣ）。用ＷＨＯＮＥＴ－３计算机软件完成数据分析。主要革兰氏阴性杆菌是铜绿假单胞菌（３２．５％）、大肠杆菌（２２．９％）、克雷伯氏菌属（１４．３％）、肠杆菌属（７．９％）、不动杆菌属（６．１％）和柠檬酸菌属（４．９％）。通过ＭＩＣ测定显示大部分菌株对１６种抗菌药物有不同程度的耐药性。４７．２％的菌株同时对５种以上抗菌药物耐药。１０６３株革兰氏阴性杆菌对亚胺培南／西司他丁最敏感，其次是对阿米卡星和头孢他啶，敏感率分别为９５％、８８％和８６％。亚胺培南／西司他丁的体外抗菌活性最高，ＭＩＣ５０和ＭＩＣ９０为０．５和２μｇ／ｍｌ，阿米卡星（ＭＩＣ５０和ＭＩＣ９０为２和６４μｇ／ｍｌ）及头孢他啶（ＭＩＣ５０和ＭＩＣ９０为２和３２μｇ／ｍｌ）次之。根据对１６种药物抗菌活性的比较，为正确合理使用抗菌药物治疗免疫功能低下者革兰氏阴性杆菌急危重症感染提供了依据
A total of 1063 Gram-negative bacilli isolated from immunocompromised patients in Wuhan were tested for their minimum inhibitory concentration (MIC) by agar plate dilution method. Complete the data analysis with WHONET-3 computer software. The major Gram-negative bacilli were Pseudomonas aeruginosa (32.5%), Escherichia coli (22.9%), Klebsiella spp (14.3%), Enterobacter spp (7.9% , Acinetobacter (6.1%) and Citrobacter (4.9%). By MIC measurement, most of the strains showed different degrees of resistance to 16 kinds of antibacterials. 47.2% strains were resistant to more than 5 antibiotics at the same time. 1063 strains of Gram-negative bacilli were the most sensitive to imipenem / cilastatin, followed by amikacin and ceftazidime, with sensitivity rates of 95%, 88% and 86%, respectively. Imipenem / cilastatin exhibited the highest in vitro antibacterial activity with MIC50 and MIC90 of 0.5 and 2 μg / ml, amikacin (MIC50 and MIC90 of 2 and 64 μg / ml) and ceftazidime (MIC50 and MIC90 of 2 And 32 μg / ml) followed. According to the comparison of the antibacterial activity of 16 kinds of drugs, it provides a basis for the correct and rational use of antibacterial drugs for the treatment of Gram-negative bacilli in critically ill patients with immunocompromised