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目的采用常压密闭缺氧耐受力实验联合急性减压缺氧耐受力实验,探索天麻素对模拟高原缺氧小鼠的保护作用。方法 100只雄性BalB/c小鼠随机分为两组用于两个实验,每组50只小鼠按照随机数字表法分为5个亚组:空白模型(M)组、红景天阳性对照(RC)组、天麻素低剂量(GAS-L)组、天麻素中剂量(GAS-M)组、天麻素高剂量(GAS-H)组,各10只。每日定时灌胃,连续7d。第七天给药50min后,分别比较5组小鼠在密闭缺氧环境下的存活时间以及在急性减压条件下的存活率,从动物整体水平上评价天麻素的抗缺氧活性。结果天麻素3个剂量均可有效延长常压密闭缺氧环境下小鼠的存活时间(P<0.01),降低小鼠暴露于急性减压缺氧条件中的死亡率。但天麻素低、中、高剂量组间无显著量效关系。结论天麻素能够对模拟高原缺氧小鼠起到一定保护作用。
Objective To investigate the protective effects of gastrodin on simulated anoxic hypoxia in mice by using a combination of hypobaric hypoxia tolerance test and acute hypobaric hypoxia tolerance test. Methods 100 male BalB / c mice were randomly divided into two groups for two experiments. Each group of 50 mice were divided into 5 subgroups according to random number table: blank group (M) group, Rhodiola rosea positive control (GAS-L), Gastrodin medium dose (GAS-M) group and Gastrodin high dose (GAS-H) group. Daily regular gavage, continuous 7d. After the fifth day of administration on the seventh day, the survival time of the five groups of mice under hypoxic environment and the survival rate under the condition of acute decompression were compared respectively. The anti-hypoxic activity of gastrodin was evaluated from the animal’s whole level. Results All three doses of gastrodin could effectively prolong the survival time of mice in anaerobic conditions (P <0.01), and reduce the mortality of mice exposed to acute hypobaric hypoxia. However, gastrodin low, medium and high dose groups no significant dose-effect relationship. Conclusion Gastrodin can protect the plateau hypoxia mice.