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目的:采用中药血清药理学的方法,观察逍遥丸含药血清(XCS)对成骨细胞增殖影响及其是否与雌激素受体相关。方法:常规方法制备XCS和妊马雌酮含药血清(CE)冻存备用,体外培养UMR-106细胞,将细胞分为空白组、空白血清组、CE组、XCS不同浓度组,MTT法检测细胞增殖率,同时观察雌激素受体拮抗剂与细胞共孵育后细胞增殖的变化。结果:与空白血清组相比,XCS四个浓度对UMR-106细胞的增殖有明显促进作用(p<0.05);但在ICI 182780干预下对细胞增殖率则无明显影响。结论:XCS对UMR-106细胞的增殖有明显促进作用,此作用依赖雌激素受体才能发挥。
OBJECTIVE: To observe the effect of Xiaoyao Pill containing serum (XCS) on the proliferation of osteoblasts and whether it is related to the estrogen receptor by using the method of traditional Chinese medicine serum pharmacology. Methods: UCS-106 cells were prepared in vitro by conventional methods. The cells were divided into three groups: blank group, blank serum group, CE group and XCS group. MTT assay Cell proliferation rate, while observing the estrogen receptor antagonist and cell co-incubation of cell proliferation changes. Results: Compared with the blank serum group, the four concentrations of XCS significantly promoted the proliferation of UMR-106 cells (p <0.05). However, ICI 182780 had no effect on the proliferation of UMR-106 cells. Conclusion: XCS can significantly promote the proliferation of UMR-106 cells, and this effect depends on the estrogen receptor.