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中国水仙用双鳞片切块繁殖法,一个切块可形成1～3个小鳞茎。小鳞茎发生于切块的两鳞片之间或外侧的茎盘上。组织学观察表明,培养前双鳞片切块基部内茎盘表层组织,除细胞略小外,与周围组织并无明显的细胞学差异。经培养后,双鳞片切块基部内、外侧茎盘组织的薄壁细胞,都能发生脱分化现象,通过细胞分裂而突起,形成1～2个腋芽原基。说明小鳞茎的形成是通过切割的创伤刺激,诱导形成的腋芽原基发展而来的,并非该处原来存在的腋芽原基。切块所带的鳞片是小鳞茎形成的营养基础,鳞片的大小和数目对小鳞茎的形成有明显的影响。不定根发生于切块或小鳞茎的茎盘上。有的小鳞茎在袋装培养中就发生1～3条不定根和1～2片绿叶。生根的小鳞茎容易移栽成苗,已生根、萌叶的小鳞茎移栽到花盆土壤中生长良好。
Chinese narcissus with double-scale dicing method, a dice can form 1 to 3 bulbs. Bulblets occur on the stem disk between the two scales or on the outside of the cut. Histological observation showed that the surface tissue of the internal stem disc in the base of double-cut sclerotia was not significantly different from the surrounding tissues except for the slightly smaller cells. After culturing, the dedifferentiation of parenchyma cells inside and outside of the double stalk dorsal base can occur, and the axon bud primordia are formed by cell division. Explain the formation of bulblets is through the cutting of the wound stimulation, inducing the formation of axillary bud primordia developed from, not where the original axillary bud primordia. Scales with scales is the basis of the formation of nutrition bulb, the size and number of scales on the formation of bulblets have a significant impact. Adventitious roots occur on the stems of cut or bulblets. Some bulbs in the bag culture occurred in 1 to 3 adventitious roots and 1 to 2 green leaves. Small rooting bulb easily transplanted into seedlings, has rooting, sprouting of small bulbs transplanted to the flowerpot soil growth well.