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目的 为初步探讨TPA用于喉癌治疗的可行性 ,对TPA分别诱发Hep 2和原代喉癌细胞的凋亡作用进行了对比观察。方法 应用琼脂糖凝胶电泳及FCM技术分别对两种细胞中的凋亡细胞进行定性、定量及细胞周期检测。结果 ① 1nMTPA作用 12h ,Hep 2和原代喉癌细胞的凋亡率分别是 11.8%和 10 .14 %(P >0 .0 5 ) ;10 0nMTPA作用 2 4h ,凋亡率达峰值 ,分别为 2 7.80 %和 2 5 %(P >0 .0 5 ) ,细胞周期阻滞于G1期 ,DNA琼脂糖凝胶电泳可检出梯状带。②TPA诱发两种细胞的凋亡率具有明显的正相关 (r =0 .9674,P <0 .0 1)。结论 TPA在体外对Hep 2和原代喉癌细胞均可诱发凋亡 ,两者的凋亡率具有较好的相关性
Objective To investigate the feasibility of TPA for the treatment of laryngeal cancer and compare the apoptosis of Hep 2 cells and primary laryngeal carcinoma cells induced by TPA. Methods Agarose gel electrophoresis and FCM were used to detect the apoptotic cells in two kinds of cells qualitatively, quantitatively and cell cycle respectively. Results ① The apoptotic rates of Hep 2 and primary laryngeal carcinoma cells were 11.8% and 10.14% (P> 0.05) after treated with 1nMTPA for 12h, respectively. The apoptotic rate reached peak at 10 0nMTPA for 24 h 2 7.80% and 25% (P> 0.05), cell cycle arrest in G1 phase, DNA ladder agarose gel electrophoresis. (2) There was a significant positive correlation between the apoptosis rate of TPA-induced two cells (r = 0.9674, P <0.01). Conclusion TPA induces apoptosis in both Hep 2 and primary laryngeal carcinoma cells in vitro with a good correlation between the apoptosis rates