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目的:在前期研究基础上,进一步探索化瘀止痛片对子宫内膜异位症的药物效应,研讨作用机理。方法:将性成熟未交配过的雌性SD大鼠,手术造成自体子宫内膜移植动物模型。随机分为阳性组(丹那唑),化瘀止痛片低、高剂量组,模型组。采用HE染色和免疫组化方法对模型大鼠异位内膜组织的细胞黏附分子(ICAM-1)、基质金属蛋白酶-2及基质金属蛋白酶抑制剂-2(MMP-2、TIMP-2)、血管内皮生长因子(VEGF)、K i-67、细胞核因子κB(NF-κB)、肿瘤坏死因子(TNF-α)、Caspase-3、雌激素受体(ER)、孕激素受体(PR)进行检测。结果:HE染色光镜下显示:3用药组异位内膜的腺体明显减少,与模型组比较,有显著性差异。化瘀止痛片高剂量组ICAM-1数值降低,与模型组比较,有显著性差异。模型组TIMP-2含量最低,化瘀止痛片高剂量组、阳性组与模型组比较,有显著性差异。化瘀止痛片高剂量组VEGF数值最低,与模型组比较有显著性差异。3用药组TNF-α数值与模型组比较均有显著性差异。化瘀止痛片高剂量组Caspase-3明显升高,与模型组比较有显著性差异。化瘀止痛片高剂量组、阳性组ER数值降低,与模型组比较有显著性差异。结论:化瘀止痛片通过抑制细胞黏附分子的作用,以抑制异位内膜对腹腔的黏附;促进异位内膜的细胞凋亡;对异位内膜的血管生成有明显的抑制作用,对异位内膜引起的炎性反应有明显的抑制作用,促进异位内膜的萎缩;降低异位内膜的雌激素受体,多层次地促使异位内膜萎缩。
OBJECTIVE: To further explore the drug effects of Huatanzhitong tablets on endometriosis on the basis of previous studies, and discuss the mechanism of action. METHODS: Sexual maturation-unpaired female Sprague-Dawley rats were surgically induced into autologous endometrial grafts. Randomly divided into positive group (danazol), Huatanzhitong tablets low, high dose group, model group. HE staining and immunohistochemistry were used to investigate the effects of ICAM-1, MMP-2, and MMP-2, TIMP-2 on the ectopic endometrial tissue of model rats. Vascular endothelial growth factor (VEGF), K i-67, Nuclear factor kappa B (NF-κB), Tumor necrosis factor (TNF-α), Caspase-3, Estrogen receptor (ER), Progesterone receptor (PR) Conduct the test. RESULTS: Light microscopy under HE staining showed that the ectopic endometrium glands in the 3 medication groups were significantly reduced, and there was a significant difference compared with the model group. The number of ICAM-1 in the high-dose group of Huatanzhitong tablets decreased, and there was a significant difference compared with the model group. The content of TIMP-2 in the model group was the lowest, and there was a significant difference between the high-dose group and the positive group of the Huatanzhitong tablets compared with the model group. The VEGF value of the high-dose group of Huatanzhitong tablets was the lowest, which was significantly different from that of the model group. 3 The TNF-α values in the drug group were significantly different from those in the model group. The Caspase-3 in the high-dose group of Huatanzhitong Tablets was significantly higher than that in the model group. The ER value of the high-dose group and the positive group of Huatanzhitong tablets decreased, and there was a significant difference compared with the model group. Conclusion: Huatanzhitong Tablets inhibit the adhesion of ectopic endometrium to the abdominal cavity by inhibiting the action of cell adhesion molecules; promote apoptosis of ectopic endometrium; and significantly inhibit angiogenesis of ectopic endometrium. The ectopic endomembrane-induced inflammatory reaction has a significant inhibitory effect, promoting ectopic endometrial atrophy; decreasing ectopic endometrial estrogen receptors, multi-level ectopic endometrial atrophy.