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应用生物技术对培矮 6 4S核心种子进行花药培养 ,获得了 91个加倍单倍体植株。在福州 (2 6°0 5′N)自然条件下对花培后代的H2 、H3、H4进行连续的育性追综观察。结果表明 ,(1 )H2 代株系间育性发生分离 ,可以把它们简单地归为三类 :完全不育 (简称培矮 6 4HS) ,不育期间其花粉不育度均在 99 8%以上 ,且不育期长 ,育性稳定 ;基本可育 (培矮 6 4HF) ,可染花粉率很高 ,几乎没有稳定不育期 ;中间类型 (培矮 6 4HM) ,育性波动大 ,不育期较短。 (2 )培矮6 4HS、培矮 6 4HF和培矮 6 4HM在H3、H4代各自的育性表现与在H2 代的育性表现一致。 (3)培矮 6 4HS比培矮 6 4S核心种子具有不育期更长 ,不育性更稳定以及更高的不育度。说明培矮 6 4S核心种子中有关育性的基因型并不纯合 ,花药培养对培矮 6 4S的遗传纯化是有效的
Biotechnology was used to culture Pei’ai 6 4S core seed in anther culture and 91 doubling haploid plants were obtained. In Fuzhou (2 6 ° 0 5’N) under natural conditions on the flower culture progeny of H2, H3, H4 continuous fertility chase observation. The results showed that: (1) The fertility of H2 lines was segregated and could be classified into three categories: sterile (Pei’ai 6 4HS), sterility of 99 8% (Pei’ai 6 4HF). The rate of dyeable pollen was very high with almost no stable period. The middle type (Peihwa 6 4HM) had large fertility fluctuation, Short period of sterile. (2) The fertility performance of Pei’ai 6 4HS, Pei’ai 6 4HF and Pei’ai 6 4HM in H3 and H4 generations was consistent with the fertility in H2 generation. (3) Pei’ai 6 4HS Peihuai 6 4S core seed has longer sterile period, more stable sterility and higher sterility. The results showed that the genotypes of fertility in Pei’ai 6 4S core seed were not homozygous, and anther culture was effective for the genetic purification of Pei’ai 6 4S.