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目的观察电针对功能性消化不良(functional dyspepsia,FD)大鼠血管活性肠肽(vasoactive intestinal peptide,VIP)、降钙素基因相关肽(calcitonin gene-related peptide,CGRP)、胃排空及小肠推进率的影响。方法将48只SD大鼠随机分为空白组、模型组及电针组,每组16只。除空白组外,模型组与电针组大鼠均采用郭氏夹尾刺激法联合不规则饮食法及冰生理盐水灌胃法多因素干预法造模,共14天。造模成功后电针组大鼠取“足三里”、“太冲”穴电针干预,每天1次,干预28天。治疗结束后分别对3组大鼠进行灌胃处理,解剖取材,测定胃内残留率及小肠推进率;观察大鼠胃窦及空肠病理变化;采用Real-time PCR法测定各组大鼠胃窦、空肠组织VIP及CGRP mRNA表达水平。结果 3组大鼠组织均未发现器质性改变,胃肠无溃疡及炎性浸润和腺上皮病变等特征。与空白组比较,模型组大鼠胃内残留率明显升高,小肠推进率降低,胃窦及空肠组织VIP及CGRP mRNA表达水平明显升高(P<0.01,P<0.05);与模型组比较,电针组大鼠胃内残留率明显降低,小肠推进率明显升高(均P<0.01),胃窦及空肠组织VIP及CGRP mRNA表达降低(P<0.05,P<0.01)。结论电针治疗可降低FD模型大鼠胃肠道VIP及CGRP表达,加速胃排空及小肠推进率。电针改善FD胃肠动力可能与降低胃肠道VIP、CGRP水平有关,提示脑肠肽分泌异常可能是FD发病的重要机制之一。
Objective To observe the effects of electroacupuncture on vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), gastric emptying and intestinal propulsion in functional dyspepsia (FD) Rate of impact. Methods 48 SD rats were randomly divided into blank group, model group and electroacupuncture group, with 16 rats in each group. Except for the blank group, rats in model group and electro-acupuncture group were all treated with Kuo-jia-tailed stimulation combined with irregular diet and ice-saline intragastric administration for 14 days. After the model was established, the rats in the electroacupuncture group took “Zusanli” and “Taichong” electroacupuncture intervention once a day for 28 days. After the treatment, the rats in each group were gavaged, the samples were taken out, the gastric residual rate and intestinal propulsion rate were measured. The pathological changes of gastric antrum and jejunum were observed. The gastric antrum , VIP and CGRP mRNA expression in jejunum. Results No organic changes were found in the three groups of rats, with no ulceration of gastrointestinal tract, inflammatory infiltration and glandular epithelial lesions. Compared with the blank group, the gastric residual rate in model group was significantly increased, the rate of small intestine was decreased, VIP and CGRP mRNA expression in gastric antrum and jejunum was significantly increased (P <0.01, P <0.05), compared with the model group (P <0.01). The VIP and CGRP mRNA expressions in gastric antrum and jejunum decreased (P <0.05, P <0.01). Conclusion Electroacupuncture can reduce the expression of VIP and CGRP in gastrointestinal tract of FD rats and accelerate the rate of gastric emptying and intestinal propulsion. Electroacupuncture to improve FD gastrointestinal motility may reduce gastrointestinal VIP, CGRP levels, suggesting that abnormal secretion of brain gut peptides may be one of the important pathogenesis of FD.