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目的:探讨中国苏南地区汉族人群不稳定型心绞痛(unstable angina pectoris,UAP)患者C-反应蛋白(C-reactive protein,CRP)基因+1444C/T多态性与其血清CRP水平的相关性。方法:本研究包括172例不稳定型心绞痛患者(UAP组)和230例非冠心病者(对照组),分别应用酶联免疫吸附法和聚合酶链反应-限制性片段长度多态性法检测血清CRP水平及CRP基因+1444C/T多态性。结果:①本研究人群存在CRP基因+1444C/T多态性;②UAP患者血清CRP水平(8.269/9.179μg/ml)显著高于非冠心病者(3.578/2.236μg/ml)(P<0.001),且经多因素Logistic回归校正各影响因素后,仍提示血清CRP水平与UAP发病风险相关;③血清CRP水平在UAP组与对照组各基因型之间无显著差异(P>0.05),进一步按性别和年龄分组后,仍未发现该基因多态性不同基因型间的血清CRP水平有任何差异(P>0.05)。结论:中国苏南地区汉族人群CRP+1444C/T基因多态性与血清CRP水平无相关性。
Objective: To investigate the association between C + reactive protein (CRP) gene + 1444C / T polymorphism and serum CRP level in patients with unstable angina pectoris (UAP) in the Han population of southern Jiangsu province. METHODS: This study included 172 patients with unstable angina (UAP group) and 230 patients with non-coronary heart disease (control group). The patients were tested by enzyme-linked immunosorbent assay and polymerase chain reaction-restriction fragment length polymorphism Serum CRP level and CRP gene +1444 C / T polymorphism. Results: ①The CRP gene + 1444C / T polymorphism existed in this study population. ② The serum CRP level in patients with UAP (8.269 / 9.179μg / ml) was significantly higher than that in non-coronary heart disease patients (3.578 / 2.236μg / ml) , And after multivariate Logistic regression to correct each influencing factor, it still suggested that serum CRP level was associated with the risk of UAP; ③ Serum CRP level had no significant difference between UAP group and control genotype (P> 0.05) There was no difference in serum CRP levels among different genotypes of this gene after genotyping by sex and age (P> 0.05). Conclusion: There is no correlation between CRP + 1444C / T polymorphism and serum CRP level in Han population of southern Jiangsu Province.