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目的探讨nm23-H1、PTEN在肝细胞癌中的表达及其与患者的临床病理特点的关系。方法应用免疫组化S-P方法,检测50例肝癌组织中nm23-H1、PTEN的表达,分析其与临床病理之间的关系。结果 nm23-H1、PTEN蛋白阳性表达为棕黄色,定位于细胞浆。10例正常肝组织和50例癌旁肝组织均可见阳性表达。肝癌组织nm23-H1、PTEN阳性率表达分别为60%(30/50)、54%(27/50),明显低于癌旁肝细胞阳性率表达100%(50/50,P<0.001,P<0.001)。nm23-H1和PTEN表达与肝癌病理分级、临床分期、肝内转移密切相关,而与患者性别、年龄、肝癌癌灶大小、肝包膜有无、甲胎蛋白水平无明显相关性。结论肝癌组织中nm23-H1、PTEN表达减低与肝癌发生发展有相关性。检测nm23-H1、PTEN两种蛋白在肝癌组织中的表达能反映肝癌的恶性程度及侵袭转移能力。
Objective To investigate the expression of nm23-H1 and PTEN in hepatocellular carcinoma (HCC) and its relationship with clinicopathological features. Methods Immunohistochemical S-P method was used to detect the expression of nm23-H1 and PTEN in 50 HCC tissues and to analyze the relationship between them and the clinicopathological features. Results nm23-H1, PTEN protein expression was brown, located in the cytoplasm. Ten cases of normal liver tissue and 50 cases of adjacent liver tissue were positive for expression. The positive expression rates of nm23-H1 and PTEN in hepatocellular carcinoma tissues were 60% (30/50) and 54% (27/50) respectively, which were significantly lower than those in paracancerous hepatocellular carcinoma tissues (50/50, P <0.001, P < <0.001). The expression of nm23-H1 and PTEN was closely related to the pathological grade, clinical stage and intrahepatic metastasis of HCC, but not with the gender, age, size of HCC, the presence of hepatic capsule and the level of alpha-fetoprotein. Conclusion The decrease of nm23-H1 and PTEN expression in HCC is correlated with the occurrence and development of HCC. Detection of nm23-H1, PTEN two proteins in the expression of liver cancer can reflect the degree of malignancy and invasion and metastasis of liver cancer.