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目的:探讨小鼠微量圆形精子细胞的冷冻方法和条件。方法:比较玻璃化冷冻和常规慢冷冻,不同浓度冷冻保护剂(5%、7%、9%甘油)及不同平衡时间(0、15、30、45、60 min)对体外培养所获微量小鼠圆形精子细胞的冷冻效果。结果:玻璃化冷冻和常规慢冷冻在7%甘油中平衡时间30 min均可获得较高的细胞复苏率,且玻璃化冷冻小鼠圆形精子细胞的复苏率显著高于常规慢冷冻方法[(72.9±15.4)%vs.(58.2±17.7)%,P<0.05]。结论:采用玻璃化冷冻方法,在7%浓度的甘油保护剂中平衡30 min,可获得较满意的微量圆形精子细胞冷冻效果。
Objective: To investigate the methods and conditions of freezing round micro-sperm cells in mice. Methods: Comparison of vitrification and conventional slow freezing, different concentrations of cryoprotectants (5%, 7%, 9% glycerol) and different balance time (0,15,30,45,60 min) Frozen effect of rat circular sperm cells. Results: The cell resuscitation rate could be obtained by vitrification and conventional slow freezing in 7% glycerol for 30 min, and the recovery rate of round sperm cells in vitrified mice was significantly higher than that of the conventional slow freezing method [( 72.9 ± 15.4)% vs (58.2 ± 17.7)%, P <0.05]. CONCLUSION: By using the vitrification method, a more satisfactory micro-circular round sperm cell freezing effect can be obtained by equilibration for 30 min in a 7% glycerol preservative.