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Objective To discuss the antitumor mechanism preliminarily by observing effects of Celastrus orbiculatus extracts(COE) on vascular endothelial growth factor(VEGF) mRNA and protein expression in hepatoma(Hepa1-6) cells of mice.Methods Hepa1-6 cells were treated with COE at different nontoxic concentration(0,10,20,40,80,and 160 μg/mL) for 16 h.The mRNA and protein expressions of VEGF were detected by reverse transcription-PCR and Western Blotting,respectively.Results COE significantly inhibited VEGF expression at both mRNA and protein levels in a dose-dependent manner.Conclusion COE can inhibit VEGF expression in Hepa1-6 cells,therefore suggest that VEGF could be chosen as an therapeutic target for COE in the context of cancer chemoprevention and anticancer therapy.
Objective To discuss the antitumor mechanism preliminarily by observing effects of Celastrus orbiculatus extracts (COE) on vascular endothelial growth factor (VEGF) mRNA and protein expression in hepatoma (Hepa 1-6) cells of mice. Methods Hepa 1-6 cells were treated with COE at different nontoxic concentrations (0, 10, 20, 40, 80 and 160 μg / mL) for 16 h.The mRNA and protein expressions of VEGF were detected by reverse transcription-PCR and Western Blotting, respectively. Results COE significantly inhibited VEGF expression at both mRNA and protein levels in a dose-dependent manner. Confocal COE can inhibit VEGF expression in Hepa 1-6 cells, therefore suggest that VEGF could be chosen as an therapeutic target for COE in the context of cancer chemoprevention and anticancer therapy.