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目的:研究苦瓜提取物对蛋白质非酶糖基化终末产物(AGE)生成的抑制作用。方法:体外蛋白质非酶糖基化反应系统中分别加入不同质量浓度的苦瓜提取物(0.01,0.1,1.0 g·L-1)或氨基胍(4 mmol·L-1),在不同时间分别测定蛋白质非酶糖化终末产物的生成量。制备糖尿病小鼠模型,以苦瓜提取物10,30 g·kg-1两个剂量连续ig 14 d,测定苦瓜提取物对糖尿病小鼠血糖以及心、肝、肾组织中AGE含量、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。结果:在体外系统中,蛋白糖化产物的生成与孵育时间呈正相关,苦瓜提取物浓度依赖性抑制蛋白糖化终末产物的生成(P<0.01)。动物实验表明苦瓜提取物能抑制血糖升高(P<0.05,P<0.01),改善糖尿病小鼠心、肝、肾组织中AGE含量(P<0.05,P<0.01)、升高SOD活性(P<0.05,P<0.01)和降低MDA含量(P<0.05,P<0.01),呈现剂量效果正相关。结论:苦瓜提取物体内体外均可有效抑制蛋白糖化终末产物的生成。
Objective: To study the inhibitory effect of bitter melon extract on the formation of non-enzymatic glycation end products (AGE). Methods: Different concentrations of Momordica charantia extract (0.01,0.1,1.0 g · L-1) or aminoguanidine (4 mmol·L-1) were added into the protein non-enzymatic glycosylation system in vitro. The amount of non-enzymatic glycation end products of protein. Diabetic mice model was prepared. With two doses of bitter melon extract 10,30 g · kg-1 for ig 14 d, the contents of AGE, superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results: In vitro system, the formation of protein glycosylation products was positively correlated with the incubation time. The extract of bitter melon inhibited the production of glycated end products in a concentration - dependent manner (P <0.01). Animal experiments showed that extract of Momordica charantia could inhibit the increase of blood glucose (P <0.05, P <0.01), and improve the activity of AGE in heart, liver and kidney of diabetic mice (P <0.05, P <0.01) <0.05, P <0.01) and decreased MDA content (P <0.05, P <0.01), showing a positive correlation between dose effects. Conclusion: Balsam pear extract can effectively inhibit the formation of glycated end products in vitro and in vivo.