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肝癌动物模型有助于肝癌发病过程中分子机制的研究。二乙基亚硝胺(DEN)是一种众所周之的化学致癌剂,已被广泛用于动物肝癌模型的建立。藻毒素MC-LR是一种由蓝藻产生的环状七肽毒素,其低浓度的慢性毒性具有潜在的促癌作用。该实验以DEN联合不同浓度的藻毒素MC-LR通过腹腔注射共同诱发大鼠肝癌模型。从形态学、病理学角度分别观察大鼠肝脏外观和肝细胞的变化;从蛋白质水平运用免疫印迹法检测谷胱甘肽-S-转移酶(GST-Pi)的表达情况。结果表明,模型组中大鼠肝癌癌变过程大致经过肝细胞损伤期、肝细胞增生–硬化期和肝细胞癌变期三个阶段。其中,DEN联合终浓度为10μg/kg的藻毒素MC-LR处理时促癌效果最佳。该方法可作为一种新型的大鼠肝癌模型建立方法为今后的研究奠定基础。
Animal model of liver cancer contributes to the molecular mechanism in the pathogenesis of liver cancer. Diethylnitrosamine (DEN) is a well-known chemical carcinogen that has been widely used in animal liver cancer model. Cytoplasmic reticulum MC-LR is a cyclic heptapeptide toxin produced by cyanobacteria that has a potential carcinogenic effect at low concentrations of chronic toxicity. The experiment with DEN combined with different concentrations of microcystin MC-LR by intraperitoneal injection of rat liver cancer model co-induced. The changes of liver appearance and hepatocytes were observed respectively from the morphological and pathological points. The expression of glutathione-S-transferase (GST-Pi) was detected by Western blotting at the protein level. The results showed that in the model group, the carcinogenesis of hepatocarcinogenesis in rats was roughly divided into three stages: hepatocellular injury, hepatocellular proliferation-cirrhosis and hepatocellular carcinoma. Among them, DEN combined with the final concentration of 10μg / kg of microcystin MC-LR treatment when the best cancer promotion effect. This method can be used as a new method to establish rat liver cancer model and lay the foundation for future research.