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目的:探讨慢性乙型肝炎中C基因启动子(BCP)变异的临床意义。方法:采用错配PCR与限制性长度片段多态性分析(RFLP)相结合,检测35例慢性乙型肝炎患者BCP区核苷酸(nt)1762碱基A→T和1764G→A联合突变及前C区nt1896G→A终止变异。结果:在35例慢性乙型肝炎中检出BCP区T1762A1764变异8例(23％),其中6例血清HBeAg(+),2例抗HBe(+),而7例前C区A1896变异中HBeAg(+)2例,抗HBe(+)5例,未见T1762A1764变异和A1896变异同时出现者。结论:提示HBV毒株BCP区T1762A1764变异可能与前C区A1896变异不同,它的出现不足以导致HBeAg(—)型的慢性肝炎。
Objective: To investigate the clinical significance of C gene promoter (BCP) mutation in chronic hepatitis B Methods: The combination of mismatched PCR and restriction fragment length polymorphism (RFLP) analysis was used to detect the 1762 base pairs A → T and 1764G → A mutations in BCP region of 35 patients with chronic hepatitis B and Pre-C region nt1896G → A termination mutation. Results: Among 35 chronic hepatitis B patients, 8 (23%) T1762A1764 mutations were detected in BCP area, including 6 serum HBeAg (+) and 2 anti-HBe (+ (+) In 2 cases and anti-HBe (+) in 5 cases. No T1762A1764 mutation or A1896 mutation appeared at the same time. Conclusion: It is suggested that the variation of T1762A1764 in BCP region of HBV may be different from the mutation of A1896 in pre-C region, and its occurrence is not enough to cause HBeAg - type chronic hepatitis.