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用体外培养方法,观察了小鼠腹腔中性粒细胞、中性粒细胞与免疫血清、补体等因子对体外机械转变的日本血吸虫童虫的杀份作用。实验结果表明:单纯小鼠中性粒细胞对童虫无杀伤作用;但在抗体及补体参与下,对童虫的杀伤明显增强,提示中性粒细胞与血清免疫因子对童虫的杀伤有协同作用。此外,比较正常小鼠和感染小鼠中性粒细胞对童虫的杀伤能力,比较不同虫龄对中性粒细胞参与的免疫作用的敏感性,证实机械转变童虫孵育24h 后对中性粒细胞参与的免疫杀伤的抵抗力增强(P<0.01)。文中对中性粒细胞依赖抗体、补体对童虫的杀伤机理进行讨论。并提出评价血吸虫细胞免疫标准化问题的意见。
In vitro culture methods were used to observe the killing effects of neutrophils and neutrophils in the peritoneal cavity of mice and immune sera, complement and other factors on mechanically transformed Schistosoma japonicum in vitro. The experimental results show that pure mouse neutrophils have no killing effect on schistosomula; however, with the participation of antibodies and complements, the killing of schistosomia is significantly enhanced, suggesting that neutrophil and serum immune factors have synergistic effects on the killing of schistosomulae. effect. In addition, comparing the killing ability of neutrophils against schistosomula in normal mice and infected mice and comparing the sensitivities of neutrophils involved in neutrophils of different ages, it was confirmed that mechanically transformed larvae were neutralised after 24 h incubation. The immunity of the cells participating in the immune killing was enhanced (P<0.01). In this paper, the killing mechanism of neutrophil-dependent antibodies and complements on schistosomula is discussed. And put forward suggestions for evaluating the standardization of schistosome cell immunity.