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探讨脐血血清对急性白血病化疗后骨髓抑制期造血细胞增殖的促进作用。采用体外CFU-GM培养法观察正常成人血清和脐血血清对正常造血细胞和急性白血病化疗后骨髓抑制期造血细胞增殖的影响。结果显示:脐血血清能明显促进正常CFU-GM和急性白血病化疗后骨髓抑制期CFU-GM生长。因此:脐血在体外能够刺激正常骨髓和急性白血病化疗后骨髓抑制期骨髓造血祖细胞增殖,提示急性白血病强化疗和干细胞移植时输注脐血可促进骨髓造血功能恢复。
To investigate the effect of umbilical cord blood serum on the proliferation of hematopoietic cells during myelosuppression after acute leukemia chemotherapy. In vitro CFU-GM culture method was used to observe the effect of normal adult serum and umbilical cord blood serum on the proliferation of hematopoietic cells at bone marrow suppression stage after chemotherapy in normal hematopoietic cells and acute leukemia. The results showed that serum of umbilical cord blood can obviously promote the growth of CFU-GM during the bone marrow suppression period after chemotherapy of normal CFU-GM and acute leukemia. Therefore, umbilical cord blood can stimulate bone marrow hematopoietic progenitor cell proliferation in bone marrow suppression after chemotherapy in normal bone marrow and acute leukemia in vitro, suggesting that infusion of umbilical cord blood can promote bone marrow hematopoietic function recovery after acute leukemia chemotherapy and stem cell transplantation.