大鼠肝缺血腺苷预处理的作用机制

• 作 者: 陈玺华 鲍民生 李正中
• 机 构: 山西医科大学第一医院普外科
• 出 处: 世界华人消化杂志
• 发表日期: 1999年04期
• 关键词: 大鼠 肝缺血 腺苷预处理

目的探讨缺血预处理（ＩＰＣ）在肝缺血再灌注（Ｉ／Ｒ）损伤中的作用及腺苷在此过程中的作用机制．方法采用大鼠原位肝Ｉ／Ｒ模型，缺血前舌静脉注射腺苷（ａｄｅｎｏｓｉｎｅ，１００ｍｇ／ｋｇ），或硫苯茶碱（ＳＰＴ，１０ｍｇ／ｋｇ），观察血ＡＬＴ，ＡＳＴ及肝组织ＬＰＯ，ＧＳＨＰｘ和ＳＯＤ变化．结果ＩＰＣ降低了血ＡＬＴ，ＡＳＴ及肝组织自由基水平（ＡＬＴ，ｎｍｏｌ／ｓ，７８３±２００ｖｓ２８０１±２１７；ＡＳＴ，ｎｍｏｌ／ｓ，１６６７±３３３ｖｓ３１３４±６００；ＬＰＯ，μｍｏｌ／ｇ，０４５±０１１ｖｓ０６１±０１３；ＧＳＨＰｘ，ＫＥＵ／ｇ，２２２±０３０ｖｓ１５６±０２５，Ｐ＜００１）．施加腺苷后，血ＡＬＴ，ＡＳＴ及肝组织自由基水平较Ｉ／Ｒ组明显下降，呈现出类似ＩＰＣ的保护作用（ＡＬＴ，ｎｍｏｌ／ｓ，１１１７±１８３ｖｓ２８０１±２１７；ＡＳＴ，ｎｍｏｌ／ｓ，２１６７±２８３ｖｓ３１３４±６００；ＬＰＯ，μｍｏｌ／ｇ，０４６±０１１ｖｓ０６１±０１３；ＧＳＨＰｘ，ＫＥＵ／ｇ，２３４±０３５ｖｓ１５６±０２５；Ｐ＜００１）．当施加腺苷受体的阻滞剂ＳＰＴ后，ＩＰＣ的保护作用被消除（Ａ?

Objective To investigate the role of ischemic preconditioning (IPC) in hepatic ischemia-reperfusion (I / R) injury and the role of adenosine in this process. Methods The rat model of orthotopic liver I / R was used to observe the changes of ALT, AST and hepatic LPO after injection of adenosine (100 mg / kg) or thiophanophylline (SPT, 10 mg / kg) , GSH  Px and SOD changes. Results IPC reduced the levels of ALT, AST and liver tissue free radicals (ALT, nmol / s, 783 ± 200 vs 2801 ± 217; AST, nmol / s, 1667 ± 333 vs 3144 ± 600; LPO,  11vs061 ± 013; GSHPx, KEU / g, 222 ± 030vs156 ± 025, P <001). After administration of adenosine, the levels of ALT, AST and free radical in liver tissue decreased significantly compared with those in I / R group, showing protective effects similar to IPC (ALT, nmol / s, 1117 ± 183 vs 2801 ± 217; AST, nmol / s, 2167 ± 283vs3134 ± 600; LPO, μmol / g, 046 ± 011vs061 ± 013; GSHPx, KEU / g, 234 ± 035vs156 ± 025; P <0 01). The protective effect of IPC was abolished when adenosine receptor blocker SPT was applied (A?