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The polysaccharides extracted from Inula britannica flower(IBP) exhibited a significant anti-liver injury activity.The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography(HPLC) and infrared spectroscopy(IR) methods.The results show that the molecular weight distribution of IBP is from 600 to 3500.The weight-average molecular weight is 1099.IBP consists of mannose,glucuronic acid,rhamnose,galacturonic acid,glucose,galactose and arabinose with a molar ratio of 4.1:1:1.4:2.7:14.6:6.3:7.9.The IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins.After administration of IBP with 100,50 and 25 mg/kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide(BCG-LPS),not only the blood alanine aminotransferase(ALT) and aspartate aminotransferase(AST) levels decreased significantly(P<0.05),but aslo the liver tissue malondialdehyde(MDA) level decreased significantly(P<0.01).The superoxide dismutase(SOD) level increased significantly(P<0.01).IBP shows significant anti-liver injury and antioxidation activity.
The polysaccharides extracted from Inula britannica flower (IBP) exhibited a significant anti-liver injury activity. The properties and chemical compositions of IBP were analyzed via high performance liquid chromatography (HPLC) and infrared spectroscopy (IR) methods. The results show that the molecular The weight distribution of IBP is from 600 to 3500. The weight-average molecular weight is 1099. IBP consists of mannose, glucuronic acid, rhamnose, galacturonic acid, glucose, galactose and arabinose with a molar ratio of 4.1: 1: 1.4: 2.7: 14.6: 6.3: 7.9. IR spectrum of IBP reveals the typical characteristics of polysaccharides and proteins. After administration of IBP with 100, 50 and 25 mg / kg body mass to liver injury mice induced by CCl 4 or bacillus calmette guerin-lipopolysaccharide ( BCG-LPS), not only the blood alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels decreased significantly (P <0.05), but aslo the liver tissue malondialdehyde (MDA) The level of superoxide dismutase (SOD) increased significantly (P <0.01). IBP shows significant anti-liver injury and antioxidant activity.