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目的 观测双参胶囊 (Shuangshen Capsule,SSC)对小鼠实验性糖尿病的影响 .方法 采用 80 m g· kg- 1 链脲霉素 (Streptozotocin,STZ;n=1 8)和 2 0 μg· kg- 1 肾上腺素(Adr;n=1 0 )小鼠 ip复制糖尿病模型 ;动物随机分组 ,并以0 .6 ,1 .2和 2 .4g· kg- 1 SSC ig,连续 4,8和 1 2 d,末次 ig后 1 h眼眶采血 ,按邻甲苯胺法测定血糖 ;按蒽酮法测定肝糖元 .结果 1 SSC 0 .6 ,1 .2和 2 .4g· kg- 1 SSC给小鼠 ig,仅 1 2 d时高剂量组血糖由 (5 .8± 0 .6 ) m mol· L- 1 降至 (5 .4± 0 .5 ) mmol· L- 1 (P<0 .0 5 ) ;2 SSC中、高剂量组给 STZ糖尿病小鼠 ig 8d和 1 2 d时 ,其血糖分别是 (2 0 .5± 5 .0 ) ,(1 8.8± 5 .1 ) mmol· L- 1和 (1 7.7± 5 .1 ) ,(1 7.0± 4.4) mmol· L- 1 [模型组分别为 (2 3.9± 4.9) ,(2 3.4± 5 .7) mmol· L- 1 ;P<0 .0 1 ];Adr糖尿病小鼠是 (1 1 .2± 1 .5 ) m mol· L- 1 ,中、高剂量组 SSC ig后 ,其血糖分别降至 (9.5± 0 .8)和 (9.0±1 .6 ) mmol· L- 1 (P<0 .0 1 ) . 3低、中和高剂量 SSC均使正常小鼠肝糖元显著增高 (P<0 .0 1 ) ,并降低 Adr糖尿病小鼠口服糖负荷后血糖峰值 (P<0 .0 5或 0 .0 1 ) .结论 SSC降血糖作用机制可能与其促进胰岛素分泌或增加组织对糖转
Objective To observe the effect of Shuangshen Capsule (SSC) on experimental diabetes in mice. Methods 80 mg·kg-1 streptozotocin (STZ; n=1 8) and 20 μg·kg-1 were used. Adrenergic (Adr;n=1 0) mice were ip replicated in a diabetic model; animals were randomly divided into groups of 0.6, 1.2, and 2.4 g.kg-1 SSC ig for 4, 8, and 12 consecutive days. Blood was collected from the eyelids 1 h after the last ig and blood glucose was measured by the o-toluidine method. Hepatic glycogen was measured by the anthrone method. Results 1 SSC 0.6, 1.2, and 2.4 g. On the 2nd day, the blood glucose decreased from (5.8 ± 0.6) mol · L - 1 to (5.4 ± 0.5) mmol · L - 1 (P <0.05); When the SSC medium and high dose groups gave STZ diabetic mice ig 8d and 12d, their blood glucose levels were (20.5 ± 5.0%), (18.8 ± 5.1) mmol · L-1, and (1) 7.7±5.1, (17.0±4.4) mmol·L-1 [model group, (23.9±4.9), (23.4±5.7) mmol·L-1; P<0.01. The Adr diabetic mice were (1 1.2±1.5) mmol·L-1. After SSC ig in the middle and high dose groups, the blood glucose decreased to (9.5±0.8) and (9.0±1) respectively. .6 ) mmol· L - 1 (P<0 .0 1 ). 3 Low, Neutral, and Neutral High-dose SSC significantly increased the hepatic glycogen content in normal mice (P<0.01), and decreased the peak glucose level in oral glucose load in Adr diabetic mice (P<0.05 or 0.01). Conclusion SSC Hypoglycemic mechanism may be related to its promotion of insulin secretion or increased tissue-to-sugar transfer