论文部分内容阅读
目的：观察心肺复苏后狗脑组织Ｃａ２＋Ｍｇ２＋ＡＴＰ酶活性的改变，探讨其对脑复苏的意义。方法：静脉注射１０％氯化钾造成心搏、呼吸骤停３０分钟后，分２组进行心肺复苏，每组６只狗；第１组用传统方法，第２组用心肺转流（ＣＰＢ）法，３０分钟后观察复苏效果，并于复苏后第１０、１５、２０、２５、３０和１２０分钟时开颅，分别取大脑皮层和海马区组织，行脑组织Ｃａ２＋Ｍｇ２＋ＡＴＰ酶测定。另外，取２只正常狗脑组织作为对照。结果：第１组仅１只狗于复苏第１５分钟后一度恢复自主循环，２０分钟后瞳孔开始缩小，但３０分钟后，瞳孔再次散大，至复苏后２５分钟心搏再次停止；其余５只均未能恢复自主循环。第２组６只狗全部恢复自主循环，瞳孔缩小到正常大小，并出现对光反应。复苏后测脑组织Ｃａ２＋Ｍｇ２＋ＡＴＰ酶活性，在第１组呈递减趋势，而第２组则呈上升趋势，直至正常。２组海马区的酶活性均高于皮层区（Ｐ＜０．０１）。结论：狗心搏、呼吸骤停３０分钟后，用ＣＰＢ方法复苏，其效果明显优于常规法；心肺复苏后，其脑组织Ｃａ２＋Ｍｇ２＋ＡＴＰ酶活性的恢复将有利于改善脑细胞内Ｃａ２＋超载，阻止脑细胞不可逆死亡，恢复脑细胞功能，从而达到脑复苏。海马区比皮层区更容易复苏?
OBJECTIVE: To observe the changes of Ca2 + Mg2 + ATPase activity in dog brain tissue after cardiopulmonary resuscitation (CPR) and to explore its significance on cerebral resuscitation. Methods: Cardioplegia was induced by intravenous injection of 10% potassium chloride. Cardiopulmonary resuscitation was performed 30 min after respiratory arrest, and 6 dogs in each group were given CPR. Group 1 was treated with CPB, Method. After 30 minutes, the resuscitation effect was observed. At the 10th, 15th, 20th, 25th, 30th and 120th minute after resuscitation, the craniotomy was performed. The contents of Ca2 + Mg2 + ATPase in the cerebral cortex and hippocampus were measured respectively. In addition, take two normal dog brain tissue as a control. Results: Only 1 dog in Group 1 regained spontaneous circulation once 15 minutes after resuscitation. After 20 minutes, the pupil began to shrink. However, after 30 minutes, the pupil dilated again and stopped again at 25 minutes after resuscitation. The remaining 5 Failed to resume their own cycle. Group 2 6 dogs all return to spontaneous circulation, the pupil shrinks to normal size, and the light response. After resuscitation, the activity of Ca2 + Mg2 + ATPase in brain tissue was decreased in group 1, while in group 2, the tendency was upward until normal. The enzyme activity of hippocampus in both groups were higher than that in cortex (P <0.01). CONCLUSION: After 30 minutes of cardiac arrest and respiratory arrest, the resuscitation by CPB method is obviously better than conventional method. Recovery of Ca2 + Mg2 + ATPase activity in brain tissue after cardiopulmonary resuscitation will be beneficial to improve intracellular Ca2 + overload and prevent brain Irreversible cell death, recovery of brain cell function, so as to achieve brain recovery. Hippocampus is easier to recover than cortical area?