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以３２Ｐ标记的甘蓝黑腐病黄单胞菌赖氨酰－ｔＲＮＡ合成酶基因为探针，将水稻白叶枯病黄单胞菌同功的基因定位于ｐＧＸＮ３０００１．６ｋｂ的ＢａｍＨＩ片段上．通过ＤＮＡ－ＤＮＡ杂交，标记置换等方法初步证实：在稻白叶枯病黄单胞菌中只有一个拷贝的赖氨酰－ｔＲＮＡ合成酶基因，该基因失活对病菌是致死的。
The 32P-labeled X. lysoyl-tRNA synthetase gene of Xanthomonas campestrinee was used as a probe to locate the homologous gene of Xanthomonas oryzae to the BamHI fragment of pGXN30001.6kb. By DNA-DNA hybridization, marker replacement and other methods initially confirmed: Xanthomonas oryzae only one copy of the lysyl-tRNA synthetase gene, the gene inactivation of the bacteria are lethal.