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目的:探讨人精液中一氧化氮(nitricoxide,NO)与生殖细胞凋亡的关系。方法:采用镀铜镉还原荧光法检测NO代谢产物硝酸盐(NO-3)。用脱氧核苷酸末端转移酶(TdT)介导的缺口末端标记(TUNEL)法和透射电镜,分别检测和观察生殖细胞的凋亡及凋亡细胞的超微结构。结果:生育组精液中NO的含量为(56.83±11.65)μmol/L,生殖细胞的凋亡率(4.60±1.25)%,与不育组(128.86±23.76)μmol/L和(17.36±3.05)%相比较有非常显著性差异(P<0.01)。不育组NO的含量和生殖细胞的凋亡率呈显著的正相关(r=0.96)凋亡的生殖细胞核染色质浓缩在核周形成新月形,电子密度增高,核膜折叠,核裂解形成凋亡小体。结论:不育者精液中NO的含量与生殖细胞的凋亡率有密切关系。高浓度的NO可能是导致睾丸生殖细胞凋亡率增加而致使男性生育力下降的原因。
Objective: To investigate the relationship between nitric oxide (NO) and germ cell apoptosis in human semen. Methods: NO metabolite nitrate (NO-3) was detected by copper-cadmium reduction fluorescence method. TdT-mediated nick end labeling (TUNEL) and transmission electron microscopy (TEM) were used to detect and observe the germ cell apoptosis and ultrastructure of apoptotic cells. Results: The content of NO in fertility group was (56.83 ± 11.65) μmol / L and the germ cell apoptosis rate was (4.60 ± 1.25)%, which was significantly higher than that in sterile group (128.86 ± 23.76 μmol / L and (17.36 ± 3.05) % Compared to a very significant difference (P <0.01). There was a significant positive correlation between NO content in germ cells and the germ cell apoptosis rate (r = 0.96). The chromatin condensation of germ cells became crescent in the perinuclear area, the electron density increased, the nuclear membrane collapsed, and nuclear fragmentation was formed Apoptotic bodies. Conclusion: The content of NO in infertile semen is closely related to the germ cell apoptosis rate. High concentrations of NO may be the cause of testicular germ cell apoptosis rate increases resulting in decreased male fertility reasons.