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类病斑突变体是研究植物程序性死亡和抗病性的理想材料。为了丰富小麦斑点突变体的研究,对叠氮化钠诱变小麦品种陕农33产生的稳定遗传的白斑突变体I30进行了特征特性研究和遗传分析。结果表明,突变体I30从三叶期开始表现白色块斑和长条纹。锥虫蓝染色和DAB染色显示,I30斑点处出现细胞死亡和H_2O_2积累现象。透射电子显微镜观察表明,I30的叶绿体形状发生改变,数目减少,基粒垛叠高度无序,部分甚至降解。农艺性状调查结果表明,I30的株高、单株有效穗数、穗粒数、穗长和结实率与野生型间无显著差异,但千粒重、穗粒重、单株产量、旗叶长度和宽度显著低于野生型。遗传分析表明,I30由1对隐性核基因控制。利用BSA+660K基因芯片技术,将该基因定位于小麦6D染色体上,位于SSR分子标记Xcfd190和6DS-5之间,遗传距离分别为6.4cM和9.1cM。
Lesion-like mutants are ideal materials to study plant programmed death and disease resistance. In order to enrich wheat spot mutants, we studied the characterization and genetic analysis of stable inherited white spot mutant I30 induced by sodium azide mutagenic wheat cultivar Shannong33. The results showed that the mutant I30 showed white patches and long stripes from the trilobe stage. Trypan blue staining and DAB staining showed cell death and H 2 O 2 accumulation at the I30 spot. Transmission electron microscopy showed that the shape of chloroplast of I30 changed, the number decreased, the height of the stack was even, and some of them were degraded. The results of agronomic traits showed that there were no significant differences in plant height, number of panicles per panicle, number of spikes per panicle, spike length and seed setting rate between I30 and wild type, but grain weight, grain weight, yield per plant, flag leaf length and width Significantly lower than the wild type. Genetic analysis shows that I30 is controlled by a pair of recessive nuclear genes. The gene was mapped on chromosome 6D of wheat using the BSA + 660K gene chip and located between the SSR molecular markers Xcfd190 and 6DS-5 with genetic distances of 6.4cM and 9.1cM, respectively.