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Autofluorescence imaging (AFI) systems are widely used in the detection of precancerous lesions.Fluorescence images of precancerous tissue are usually red (R) or blue (B),so this kind of system has high requirement for colour recovery,especially in R and B channels.Besides,AFI system requires bulk data transmission with no time delay.Existing colour recovery algorithms focus more on green (G) channel,overlooking R and B channels.Although the state-of-art demosaicing algorithms can perform well in colour recovery,they often have high computational cost and high hardware requirements.We propose an efficient interpolation algorithm with low complexity to solve the problem.When calculating R and B channel values,we innovatively propose the diagonal direction to select the interpolation direction,and apply colour difference law to make full use of the correlation between colour channels.The experimental results show that the peak signal-to-noise ratios (PSNRs)of G,R and B channels reach 37.54,37.40 and 38.22 dB,respectively,which shows good performance in recovery of R and B channels.In conclusion,the algorithm proposed in this paper can be used as an altative to the existing demosaicing algorithms for AFI system.