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作者应用mRNA差异显示、DNA序列分析和Northern杂交技术，对紫杉醇处理人乳癌细胞诱导的表达基困进行cDNA克隆研究，12个克隆有明确的mRNA水平表达改变，其中6个cDNA克隆核旮酸序列与已知的cDNA序列有较高的同源性，其余6个尚无同源性基因。克隆C3P3与人腺苷基蛋氨酸合成酶基因序列同源性达99％，紫杉醇诱导该酶基因转录活动和基因产物活性增高。提示mRNA差异显示技术在筛选mRNA水平表达差异的基因cDNA克隆研究中是可行的和有效的。
Using mRNA differential display, DNA sequence analysis and Northern hybridization techniques, the authors performed a cDNA cloning study on pachyclitol-treated human breast cancer cells to induce expression gaps. Twelve clones had clear mRNA expression changes, including six cDNA clones. There is a high homology with known cDNA sequences, and there are no homologous genes in the remaining six. The homology of cloned C3P3 with the human adenosyl methionine synthetase gene sequence was 99%. Paclitaxel induced transcription activity and gene product activity of the enzyme. It is suggested that mRNA differential display technology is feasible and effective in the screening of cDNA clones for screening differential mRNA levels.