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用人类红白血病细胞(K562)为材料,通过细胞计数、MTT法测定,形态学观察、NBT还原试验及联苯胺反应等方法检测了不同浓度姜黄素对体外人K562细胞的作用。结果表明,姜黄素对K562细胞有明显的抑制作用,随浓度增加,抑制作用逐渐增强。形态学观察可见轻微诱导分化作用。鉴于姜黄素对人类红白血病细胞兼有轻微诱导分化和增殖抑制作用,提示姜黄素有可能应用于人类红白血病的治疗。
Using human erythroleukemia cells (K562) as material, the effects of different concentrations of curcumin on human K562 cells were examined by cell counting, MTT assay, morphology observation, NBT reduction test and benzidine reaction. The results showed that curcumin had a significant inhibitory effect on K562 cells, and the inhibitory effect gradually increased with increasing concentrations. Morphological observations showed slight induction of differentiation. In view of curcumin on human erythroleukemia cells have a slight induction of differentiation and proliferation inhibition, suggesting that curcumin may be applied to the treatment of human erythroleukemia.